《植物生理学报》 2015, 51(1): 79-87
通信作者:于;E-mail: wmzhu69@hotmail.com;Tel: 021-62208660
摘 要:
促分裂原活化蛋白激酶(MAPK)信号系统在植物应对环境胁迫中发挥重要作用。为了探讨LeMPK3基因在低温胁迫中的功能, 我们从番茄中分离了LeMPK3基因并将其转化到烟草中, 获得了异源表达LeMPK3基因的转基因烟草。RT-PCR分析结果表明, 番茄幼苗在低温和高温胁迫及外源物质——过氧化氢(H2O2)、茉莉酸甲酯(MeJA)和水杨酸(SA)处理下LeMPK3基因的表达量提高。在烟草中异源表达LeMPK3基因可以提高超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性, 增加脯氨酸和可溶性糖的含量, 增强逆境相关基因的表达。说明异源表达番茄LeMPK3基因提高烟草对低温的抗性。关键词:番茄; 低温胁迫; 转基因; LeMPK3
收稿:2014-10-30 修定:2015-01-06
资助:上海市农科院青年科技发展基金(农科青年科技2012-06)、浦东新区科技发展基金创新资金(PKQ2012-03)、公益性行业(农业)科研专项(201403032)。 * 共同第一作者。
Corresponding author: YU Li; E-mail: wmzhu69@hotmail.com; Tel: 021-62208660
Abstract:
Mitogen-activated protein kinase (MAPK) cascades are important intracellular signaling events that mediate signal transduction associated with the plant response to environmental stress. In order to investigate the function of LeMPK3 under chilling stress, LeMPK3 was isolated from tomato (Lycopersicon esculentum) and transgenic tobacco (Nicotiana tabacum) plants were obtained. The transcription of LeMPK3 in tomato leaves was up-regulated by low and high temperatures, and by the exogenous signaling molecules methyl jasmonate (MeJA), salicylic acid (SA) and hydrogen peroxide (H2O2). Transgenic tobacco overexpressing LeMPK3 accumulated less reactive oxygen species (ROS), more superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) activities, more proline and soluble sugar contents, and more stress-responsive genes expression, leading to enhancing low temperature stress tolerance compared to the control plants.Key words: tomato; low temperature stress; transgenic; LeMPK3
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